Two fresh species of Culicoides Latreille of the Neotropical subgenus Mataemyia Vargas are described and illustrated predicated on feminine specimens from Juruti, Par, Brazil and weighed against their similar congeners. the condition of Amazonas, so five species out of this subgenus are presently known inhabiting northern Brazil. In today’s paper, we describe and illustrate two fresh species from the municipality of Juruti, PA, predicated on females specimens gathered by CDC light trap and discuss the similarities and variations making use of their related species of the subgenus (was supplied by Wirth and Soria (1981) for just eight species contained LY2109761 irreversible inhibition in group, we upgrade the main element for the identification of the 19 species from the subgenus and produced a synopsis of the subgenus mounted on an Olympus BH-2 microscope and the plates had been ready using Photoshop GIMP Lightweight. Microphotographs of the wings had been used with a Nikon Eclipse Electronic-800. The overall terminology used can be that useful for by Felippe-Bauer SH3RF1 (2003). Conditions for structures adhere to the Manual of Central America Diptera (Dark brown et al. 2009). The measurements of the spermathecae are in micrometers and the ones of the wings are in millimetres. Meristic info is provided as range, pursuing by the suggest and amount of specimens examined. of the subgenus Vargas – Moderate to large-sized species (wing with 0.80-1.8 mm). Eye bare. Palpus moderately swollen; third segment with moderately wide pit situated on mid-size or subapical; palpal ratio (PR) 1.8-3.0. Antenna generally with transition long between proximal and distal series; sensilla coeloconica present on flagellomeres 1, (4), (5), 6-8 (except in 1, 6-12); LY2109761 irreversible inhibition antennal ratio (AR) 0.75-1.70 (usually a lot more than 1.00). Scutum darkish with prominent design of yellowish places. Wing with specific pale places; poststigmatic pale place in r3 generally as inverted L-shaped, almost isolating a little dark place behind second radial cellular; sometimes this little dark place is linked to the oblique dark range in r3, separating the poststigmatic pale places; distal pale place in r3 generally achieving the anterodistal part of wing margin; CuA1 and CuA2 generally dark; wing bottom usually with wide pale place extending from costal margin to anal cellular; macrotrichia in distal half of wing. Legs dark brown with distinct pale bands characteristics of each species; hind tibial comb with four spines (rarely 5 or 6). Two (rarely 1) spermathecae present. Male tergite 9 with long, subparallel apicolateral processes (rarely slender and well separated). Gonocoxite moderately stout, dorsal and ventral root moderately to well developed; gonostylus curved, slender. Aedeagus triangular with rounded basal arch extending 0.5 of total length, tapering to bifid or slender, LY2109761 irreversible inhibition single apex. Parameres separate; mid-portion long, slender; apical portion tapered, abruptly bent ventrally, mesally directed, with fringe of fine spicules or as simple, filiform tip. Key to the species of the Culicoides (Mataemyia) Vargas (primarily to females) Sensilla coeloconica on flagellomeres 1, 6-12………………………………………… Wirth & Blanton – Sensilla coeloconica on flagellomeres 1, (4), (5), 6-8…………………………………………………………………………… 2 One spermatheca; hind femur dark to tip; sensilla coeloconica on flagellomeres 1, 5-8…………………………… 3 – Two spermathecae; hind femur various; sensilla coeloconica on flagellomeres 1, (4), (5), 6-8……………………. 4 Mid femur dark to tip; hind tibial comb with six spines; second radial cell pale in mid portion with extreme base and tip in dark spots………………………………………………………………………………… Wirth & Blanton – Mid femur with subapical pale band; hind tibial comb with four spines; second radial cell in dark spot…………………………………………………. Spinelli Base of the wing with a pale spot restricted to the area near basal arculus……………………………………………….. 5 – Base of the wing broadly pale, sometimes extending from costal margin to anal cell…………………………………… 7 Large species, wing length 1.45 mm; one distal pale spot in anal cell; distal pale spot in r3 single, irregular, slightly meeting wing margin.