They particularly play important role in the well-being of women and children . collected regularly for antibody assay from individual chicken vaccinated with ND I2 vaccine using different routes. Results At baseline, there was no difference in antibody titer among the experimental groups. After the first and booster vaccinations, the three vaccinated groups had significantly higher Etidronate (Didronel) antibody titer ( em P /em ? ?0.001) than the unvaccinated control group. Interestingly, there was no statistically significant difference in antibody titer among the vaccinated groups. Out of the 40 chicken in the unvaccinated control only 14 had antibody titter log23. Similarly 19/37 of chicken in the drinking water group, 19/37 of chicken in the eye drop group and 20/40 chicken in the spray group had antibody titer log23. Two weeks after the first vaccination the proportion of chicken with antibody titer log23 rose to 23/37, 30/37 and 29/40 in the group vaccinated via drinking water, eye drop and spray, respectively. The proportion remained low in unvaccinated group. Hundred percent of the vaccinated chicken survived after infection with the virulent ND virus (Alemaya strain); whereas only 40% survived from the unvaccinated control group. Conclusion The results of this study showed that ND I2 vaccine administered via drinking water and spray under smallholder farmers situation provoked protective antibody level similar to the eye drop method. The use of ND I2 vaccine could contribute to food security if used by rural poultry farmers properly. RASA4 strong class=”kwd-title” Keywords: Newcastle disease, I2 vaccine, Smallholder, Village, Chicken Background Chicken production is considered an important source of quality animal proteins and can break the vicious cycle of poverty and malnutrition in developing countries. They particularly play important role in the well-being of women and children . In Ethiopia, locally produced chickens are genetically diverse  with low production , which may be attributed to ND. Increasing productivity and monetary return from yard chicken requires better knowledge of diseases such as ND. Newcastle disease can intercept the development of small level poultry farms in the rural Ethiopia unless its incidence is reduced. Distribution of various breeds and uncontrolled marketing can aid the spread of ND between and within poultry farms. For example, more than 15 outbreaks of ND were confirmed in 2016 whereas as hundreds of outbreaks were confirmed in 2015 in the country. This demonstrates ND is continuing to effect the livelihood of poultry farmers. To improve productivity and monetary return from yard flocks, improvements to education and biosecurity actions are required. Effective control of ND is needed to improve the livelihood of smallholder farmers particularly women in developing countries . Vaccination has been regarded as effective and affordable control option in several countries [4C8]. It has been widely used in commercial poultry farms. The demand for vaccine against ND has grown in smallholder and town poultry producers. However, the conventional vaccine application methods are not suitable for Etidronate (Didronel) smallholder and town poultry production systems . An innovative alternate for smallholder and town poultry producers was developed in Australia using warmth stable ND I2 disease . It has been proven to be suitable for town poultry in Asian and some African countries in the absence of chilly chain  and is cheaper than standard ND vaccines . Despite development of smallholder poultry production, info on the use of ND I2 vaccine and route of vaccine delivery with ideal protection level has not been sufficiently explored in Ethiopia. Appropriate vaccine Etidronate (Didronel) delivery route that can be used from the farmers could contribute to food security. The main objective of this study is definitely, therefore, to evaluate the effectiveness of ND I2 vaccine delivered via drinking water and aerosol. Methods Study area This study was carried out in Minjar-Shenkora area, North Shoa Zone, Amhara Regional State, Etidronate (Didronel) central Ethiopia. It is located between 906 and 905 N and 3946 and 3926 East 135?km south east of Addis Ababa (Fig.?1). The altitude of the area ranges from 1400 to 2400?m.a.s.l. The average annual rainfall of the area ranges from 62.8?mm C 1028?mm in bimodal pattern. The mean annual temp of the last 10 years ranges from 7.3?C C 20?C. Agriculture is the main stay of the economy of people inhabiting the area in which livestock production takes on key part. The livestock human population of the area comprises cattle (95270), sheep (57603), goats (74049), poultry (168,702) and equines (3826). Unique poultry such as.