*Values significantly different from percent decrease in and ?andand ?andand ?andand ?and= 11 filters). next generated a cumulative dose-response curve of the and Atopaxar hydrobromide Atopaxar hydrobromide ?and= 16C18 filters). *Value significantly different from that induced by sequential addition of PGE2 in the reverse order. = 11). *Value significantly different from addition of PGE2 to the Zfp264 apical side. Conductance values were also significantly different between cells receiving amiloride vs. amiloride and apical PGE2. = 11). Conductance after apical addition of PGE2 was not significantly different from that after basal addition (NS). To identify which class of prostanoid receptors is responsible for and ?andand ?andand ?and= 6C12 filters). *Value significantly different from that induced by other EP receptor agonists. As a complementary approach for identifying the EP receptors responsible for = 12 filters). *Value significantly different from that in cells pretreated with vehicle. = 6 filters). *Values significantly different from that in cells treated with apical L-161,982. Although we observed a sidedness to the latency period for Atopaxar hydrobromide inhibition of = 12 filters). We next used RT-PCR to evaluate EP4 receptor mRNA expression in mIMCD-K2 cells. Mouse kidney cDNA were run in parallel as a positive control. We detected all four classes of EP receptors in mouse kidney homogenates. We also found that EP4 receptors, as well as EP1 and EP2 receptors, are expressed in mIMCD-K2 cells (Fig. 7). Open in a separate window Fig. 7. Expression of EP1, EP2, EP3, and EP4 receptor mRNA in mouse kidney lysates and mIMCD-K2 cells. Photograph of a gel showing PCR amplification products in mouse kidney tissue and mIMCD-K2 cells after reverse transcription of mouse EP1, EP2, EP3, and EP4 receptor mRNA. Samples containing reverse transcriptase (RT; +) or negative controls lacking reverse transcriptase (?) are included for each tissue/primer combination. M Atopaxar hydrobromide indicates DNA size marker. PGE2 stimulates Cl? secretion through CFTR and CACC. Because ENaC activity was blocked with addition of amiloride to the apical side of cell Atopaxar hydrobromide sheets in all experiments, we concluded that and ?andand ?and= 28 filters). *Value significantly different from = 28 filters). = 9). We next evaluated the transport pathways responsible for basolateral Cl? uptake in the and ?andand ?and= 7 filters). *Value significantly different from = 7 filters). Open in a separate window Fig. 11. Effects of bumetanide and acetazolamide (Acet) on PGE2-induced = 13 filters). *Value significantly different from = 13 filters). PGE2 stimulates PKA and Ca2+-signaling pathways. Because EP4 receptors classically couple to the Gs subunit to stimulate adenylate cyclase and PKA activity, we next evaluated whether PKA activity is required for = 13 filters). *Values significantly different from = 9 filters). *Values significantly different from IscPGE2 in vehicle-treated cells. Open in a separate window Fig. 13. Effects of inhibitors of phosphatidylinositol 3-kinase, phospholipase C, and protein kinase C on PGE2-induced and ?and= 12 filters). *Value significantly different from that in amiloride-treated cells; #value significantly different from = 6 filters). *Values significantly different from = 6 filters). *Values significantly different from percent decrease in and ?andand ?andand ?andand ?and= 11 filters). *Value significantly different from that in amiloride-treated cells; #value significantly different from = 5C6 filters). *Values significantly different from = 5C6 filters). *Values significantly different from percent decrease in = 9C11 filters). *Value significantly different from that in FFA-treated cells. To rule out the possibility that CFTR activity itself might regulate CACC, we incubated mIMCD-K2 cells with CFTR-172 before addition of PGE2. We found that PGE2 could still induce an increase in = 8 filters). oocytes. Mol Pharmacol 37: 720C724, 1990 [PubMed] [Google Scholar] 72. Ye W, Zhang H, Hillas.
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