Supplementary MaterialsS1 Fig: (a) The hereditary interaction score (epsilon score) of is definitely plotted contrary to the negative from the is definitely plotted contrary to the negative from the (blue) mutants crossed with SEY6210 cells (reddish colored). Tetrad evaluation of (blue) mutants crossed with (orange). (i) Tetrad evaluation of (reddish colored) mutants crossed with (orange). (j) Tetrad evaluation of (blue) mutants crossed with (orange).(TIF) pgen.1008745.s004.tif (1.8M) GUID:?39FEA2BC-F303-4123-B165-97257D5C17D8 S5 Fig: (a) Serial dilutions of WT, cells, cells, cells and two different clones of cells on YPD plates. Control plates (remaining) and plates including 1.8 M cerulenin (right) had been used. (b) Serial dilutions of WT, cells, cells, cells and cells on YPD plates. Control plates (remaining) and plates including 0.075 M Aureobasidin A Mouse Monoclonal to Goat IgG (right) had been used.(TIF) pgen.1008745.s005.tif (433K) GUID:?E40FE966-95F8-434E-B306-0D2A4174B5FB S6 Fig: (a) Integration of MZP-54 [13C315N1]-serine into ceramides. Cells were labelled with [2H6]-inositol and [13C315N1]-serine more than 90 mins in YPD press. Lipids were analyzed and extracted via mass spectrometry. Displayed will be the levels of [13C315N1]-serine labelled ceramides of WT cells, cells, cells and cells in mol% per all recognized lipids. The common is shown in pubs. Dots match the ideals of two 3rd party tests.(TIF) pgen.1008745.s006.tif (190K) GUID:?E3BCDA5A-B9FF-4049-824A-4428C4051063 S7 Fig: (a) Tetrad analysis of (blue) mutants crossed with Gnp1-mcherry (reddish colored). (b) Tetrad evaluation of BY (blue) mutants crossed with BY GFP-Gnp1.(TIF) pgen.1008745.s007.tif (400K) GUID:?0886F94D-37AE-4024-A9E9-BBDCF80E846F S1 Data: Data collection for hereditary interactions of SER1 and SER2 in Figs ?S1 and Figs2B2B. Data extracted from .(XLS) pgen.1008745.s008.xls (291K) GUID:?E2E23B98-5C58-479F-9B7E-430341920171 S2 Data: Data arranged for [14C]-serine uptake measurements in Fig 3A. (XLSX) pgen.1008745.s009.xlsx (16K) GUID:?7A9F9060-89B0-4D22-8534-3B5AE4F16016 S3 Data: Data set for incorportation of [13C315N1]-serine in to MZP-54 the proteome in Fig 3C. (XLSX) pgen.1008745.s010.XLSX (609K) GUID:?72044316-E085-4BF9-87F6-9E35C85F681D S4 Data: Data group of free of charge intracellular [13C315N1]-serine levels in Fig 3D. (XLSX) pgen.1008745.s011.xlsx (12K) GUID:?08DFC81E-1BF4-4746-B3B1-BCD43C8553FF S5 Data: Set of all protein determined including SILAC ratios and intensities through the comparison of WT and cells in Fig 3E. (XLSX) pgen.1008745.s012.xlsx (992K) GUID:?AA3A03C0-F906-4400-9371-BCCF9642867A S6 Data: Data set of serine, glycine and lysine levels of WT and cells with and without serine presented in Figs ?Figs4C4C and S5B. (XLSX) pgen.1008745.s013.xlsx (12K) GUID:?CCBC2F6F-9BF4-49B9-A27D-6F483F3C2484 S7 Data: Source data for the quantification of colony sizes of the tetrad analysis in Fig 5D and 5E. (XLSX) pgen.1008745.s014.xlsx (13K) GUID:?F5A9DC50-9C29-45CB-B8F9-125F74B7CAFF S8 Data: Data set of long chain base levels of WT and cells with and without myriocin presented in Fig 6B. (XLSX) pgen.1008745.s015.xlsx (26K) GUID:?24D72498-B9C7-4EF8-B209-7F6367C1C9A7 S9 Data: Data set of serine (6d) and inositol (6e) labelled IPCs and ceramides presented in Fig 6B, 6D and 6E and S6 Fig. (XLSX) pgen.1008745.s016.xlsx (20K) GUID:?5DE04D2B-8A8B-4BE5-AA64-4E76527FB464 S1 Script: MATLAB script used for the flux variability analysis in SDC media. (M) pgen.1008745.s017.m (6.1K) GUID:?544B67B7-D1C1-434A-8138-274BD63AF9DE S2 Script: MATLAB script used for the flux variability analysis in YPD media. (M) pgen.1008745.s018.m (6.6K) GUID:?792E96AD-8496-4039-B90F-A2AED5BCC1CA Data Availability StatementAll relevant data are within the manuscript and its Supporting Information files. Abstract MZP-54 Sphingolipids are abundant and essential molecules in eukaryotes that have crucial functions as signaling molecules and as membrane components. Sphingolipid biosynthesis starts in the endoplasmic reticulum with the condensation of serine and palmitoyl-CoA. Sphingolipid biosynthesis is highly regulated to maintain sphingolipid homeostasis. Even though, serine MZP-54 is an essential component of the sphingolipid biosynthesis pathway, its role in maintaining sphingolipid homeostasis has not been precisely studied. Here we show that serine uptake is an important factor for the regulation of sphingolipid biosynthesis in cells. We further show that uptake of exogenous serine by Gnp1 is important to maintain cellular serine levels and observe a specific connection between serine uptake and the first step of sphingolipid biosynthesis. Using mass spectrometry-based flux analysis, we further observed imported serine.